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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: J Steroid Biochem Mol Biol. 2009 Jun 11;117(1-3):81–86. doi: 10.1016/j.jsbmb.2009.06.002

Fig. 2. Gene expression analysis for OM and LNG.

Fig. 2

(A). Detection of various Abpa and Abpbg transcripts in the OM and LNG of male and female mice by RNA-PCR analysis. Tissues from 8- to 12-week-old mice (three per group) were pooled for total RNA preparation. PCR products were analyzed on 1% agarose gels, and visualized by staining with ethidium bromide. Numbers represent various Abpa or Abpbg transcripts. The results shown are representative of two independent determinations.

(B). Real-time RNA-PCR analysis of Abpa27 expression in the OM and LNG of male mice. The values shown represent means ± SD. *, There is a significant difference between OM (n=5) and LNG (n=4) (P < 0.01).

(C). Immunoblot analysis of sABP protein expression in the OM and LNG. Tissue homogenates from OM and LNG (30 μg protein each), pooled from three 8- to 12-week-old mice, were analyzed using a polyclonal anti-sABP antibody. The size of the sABP proteins detected is indicated. The results shown are representative of two independent determinations.

(D). RNA-PCR analysis of expression of androgen receptor (AR), aromatase, and 5α-reductase type I and type II in OM and LNG of male mice. The sizes of PCR products detected are indicated. Tissues from 8- to 12-week-old mice (three per group) were pooled for total RNA preparation. The results shown are representative of two independent determinations.