. Author manuscript; available in PMC: 2010 Oct 1.

Published in final edited form as: J Steroid Biochem Mol Biol. 2009 Jun 11;117(1-3):81–86. doi: 10.1016/j.jsbmb.2009.06.002

Table 2. In vitro metabolism of T by OM and LNG microsomal preparations.

Rates of formation of three major T metabolites, 15α-hydroxy-testosterone (15α-OH-T), 15β-OH-T, and 2β-OH-T, were determined, as described in the Materials and Methods. Reaction mixtures contained 50 mM potassium phosphate buffer, PH 7.4, 10 μM T, 1 mM ascorbic acid, 0.1 mg/ml (for OM) or 0.5 mg/ml (for LNG) microsomal protein, and 1 mM NADPH. The values presented are means ± S.D. (n = 3).

Gender	Tissue	Rates of product formation

		15α-OH-T	15β-OH-T	2β-OH-T
		pmol/min/mg protein
Male	OM	3900 ± 300	300 ± 40	330 ± 50
	LNG	110 ± 20 ^a	8.8 ± 1.6 ^a	8.8 ± 2.5 ^a
Female	OM	3300 ± 260	250 ± 30	260 ± 40
	LNG	110 ± 10 ^a	8.0 ± 0.2 ^a	8.9 ± 0.7 ^a

The rates are significantly different between OM and LNG, for both sexes (P < 0.01; Student's t-test).