Skip to main content
. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: J Steroid Biochem Mol Biol. 2009 Jun 27;117(1-3):23–30. doi: 10.1016/j.jsbmb.2009.06.004

Figure 4.

Figure 4

Effects of the MAP Kinase kinase inhibitor U0126 on progestin stimulation of MnSOD expression. T47D cells were plated, grown and treated as in Figure 1A, except that U0126 dissolved in DMSO (dimethyl sulfoxide) was added at 5 micromolar 15 minutes before R5020 ( 10 nM ) was added. Final concentrations of DMSO and ethanol were 0.1%. After 24 hours of treatment, cells were harvested, extracts made and immunoblotting performed as described in Materials and Methods. 60 μg of protein was applied to each lane. Densitometry results are the average +/− SEM of 3 independent experiments. *: different from all others at p<0.05 by ANOVA followed by the Bonferrroni t-test.