Fig. 3.

Effects of 4-DAMP mustard treatment on contractions elicited to oxotremorine-M in mouse ileum. a Responses were measured in ilea from the M₃ KO mouse (open triangles) and from wild-type ileum before (open circles) and after (closed triangles) treatment with 4-DAMP mustard (40 nM) in combination with AF-DX 116 (4 μM) for 2 h followed by washing as described under “Methods.” b Responses were measured in ilea from the M₂ KO mouse before (open circles) and after (closed triangles) treatment with 4-DAMP mustard as described in a. c Same as b except that responses were measured in ilea from the M₃ KO mouse. d All responses were measured in ilea from wild-type mice that had been treated with 4-DAMP mustard as described in a. After this treatment, responses were measured in the absence (open circles) and presence of AF-DX 116 (1 μM; closed triangles) or 4-DAMP (10 nM; open triangles). Mean values ± SEM from five to seven experiments are plotted in a–d