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. 2009 Oct 6;7(10):e1000213. doi: 10.1371/journal.pbio.1000213

Figure 3. Physical association of candidate proteins with ribosomal subunits was measured by co-sedimentation and immunoblot.

Figure 3

(A) Immunoblots of fractions collected from sucrose density gradients for strains carrying TAP-tagged gene alleles. Fractions 4 and 5, 6 and 7, 7 and 8, and 9–12 correspond to the 40S, 60S, 80S, and polysome peaks in the sucrose density gradients, respectively. BY4741 is the negative control, and Tsr1-TAP and Lsg1-TAP are the positive controls for 40S subunit biogenesis factors and 60S subunit biogenesis factors, respectively. Rps3-TAP and Rpl8a-TAP show the locations of small and large ribosomal subunits in the sucrose density gradient, respectively, whereas Eno1-TAP represents the proteins that do not co-sediment with ribosomes. (B–F) show quantitation of the immunoblots for Yil091c-TAP, Bfr2-TAP, Puf6-TAP, Jip5-TAP, and New1-TAP. (G–K) show polysome profile defects for several TAP-tagged strains.