Figure 8. Synthetic ribosome biogenesis defects were observed in a double mutant trf5ΔGAL1-PAP2, suggesting that high-scoring genes not confirmed in the previous experiments may often still participate in ribosome biogenesis.

The trf5Δ mutant was cultured in YPD. GAL1-PAP2 and trf5ΔGAL1-PAP2 were first cultured in YPGal, then diluted into YPD and cultured to early logarithmic phase. Gray arrows indicate halfmer polysomes. Strong 60S biogenesis defects were observed for the double mutants, but not in either single gene mutant.