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BRMS1 decreases prometastatic and increases metastasis-suppressing miR in breast cancer cells. RNA was collected using Qiazol and the miRNeasy kits. Purified RNA (6 μl) was reverse transcribed and then amplified usin miR specific primer probes. Relative amounts were calculated using the comparative cycle threshold (CT) method. The miR amounts were normalized to small nuclear RNA-U6. Results are given as fold change relative to non-BRMS1 expressing 231 and 435 cells ± S.E.M.
