TABLE I.
TOP 25 MOST COMMONLY REGULATED MIR FOLLOWING RE-EXPRESSION OF BRMS1
| Cell line | Direction change | microRNA | Cy5:Cy3 ratio | Locus | Accession no. Sanger database |
|---|---|---|---|---|---|
| MDA-MB-231 | DOWN | hsa_miR_200a_AS | 62.190 | 1p36 | MIMAT0001620 |
| hsa_miR_200b | 16.213 | 1p36 | MIMAT0000318 | ||
| hsa_miR_380_3p | 12.441 | 14q32 | MIMAT0000735 | ||
| hsa_miR_326 | 8.200 | 11q13 | MIMAT0000756 | ||
| hsa_miR_483 | 6.864 | 11p15 | MIMAT0002173 | ||
| hsa_miR_210 | 3.964 | 11p15 | MIMAT0000267 | ||
| hsa_miR_99b | 3.580 | 19q13 | MIMAT0000689 | ||
| hsa_miR_330 | 2.945 | 19q13 | MIMAT0000751 | ||
| hsa_miR_324_5p | 2.860 | 17p13 | MIMAT0000761 | ||
| hsa_miR_324_3p | 2.769 | 17p13 | MIMAT0000762 | ||
| hsa_miR_320 | 2.684 | 8p21 | MIMAT0000510 | ||
| hsa_miR_520h | 2.621 | 19q13 | MIMAT0002867 | ||
| hsa_miR_149 | 2.614 | 2q37 | MIMAT0000450 | ||
| hsa_miR_423 | 2.553 | 17q11 | MIMAT0001340 | ||
| hsa_miR_425 | 2.552 | 3p21 | MIMAT0001343 | ||
| hsa_miR_501 | 2.478 | Xp11 | MIMAT0002872 | ||
| hsa_miR_452 | 2.430 | Xq28 | MIMAT0001635 | ||
| hsa_miR_511 | 2.414 | 10p12 | MIMAT0002808 | ||
| hsa_miR_339 | 2.336 | 7p22 | MIMAT0000764 | ||
| hsa_miR_151 | 2.299 | 8q24 | MIMAT0000757 | ||
| hsa_miR_491 | 2.126 | 9p21 | MIMAT0002807 | ||
| hsa_miR_362 | 2.064 | Xp11 | MIMAT0000705 | ||
| hsa_miR_17_3p | 2.031 | 13q31 | MIMAT0000071 | ||
| hsa_miR_18a_AS | 1.969 | 13q31 | MIMAT0002891 | ||
| MDA-MB-231 | UP | hsa_let_7a | 0.295 | 22q13 | MIMAT0000062 |
| hsa_let_7f | 0.328 | 9q22 | MIMAT0000067 | ||
| hsa_miR_128b | 0.358 | 3p22 | MIMAT0000676 | ||
| hsa_miR_30c | 0.370 | 6q13 | MIMAT0000244 | ||
| hsa_let_7g | 0.384 | 3p21 | MIMAT0000414 | ||
| hsa_miR_30a_3p | 0.385 | 6q13 | MIMAT0000088 | ||
| hsa_miR_21 | 0.386 | 17q22 | MIMAT0000076 | ||
| hsa_miR_10a | 0.396 | 17q21 | MIMAT0000253 | ||
| hsa_miR_128a | 0.408 | 2q21 | MIMAT0000424 | ||
| hsa_miR_146b | 0.429 | 10q21 | MIMAT0002809 | ||
| hsa_miR_181a | 0.434 | 1q31 | MIMAT0000256 | ||
| hsa_miR_182 | 0.434 | 7q32 | MIMAT0000259 | ||
| hsa_miR_96 | 0.435 | 7q32 | MIMAT0000095 | ||
| hsa_miR_20a | 0.436 | 13q31 | MIMAT0000075 | ||
| hsa_miR_200c | 0.436 | 12p13 | MIMAT0000617 | ||
| hsa_miR_26a | 0.463 | 3p22 | MIMAT0000082 | ||
| hsa_let_7i | 0.470 | 12q14 | MIMAT0000415 | ||
| hsa_miR_15b | 0.504 | 3q25 | MIMAT0000417 | ||
| hsa_miR_148a | 0.510 | 7p15 | MIMAT0000243 | ||
| hsa_miR_30a_5p | 0.515 | 6q13 | MIMAT0000087 | ||
| hsa_miR_15a | 0.518 | 13q14 | MIMAT0000068 | ||
| hsa_miR_27a | 0.520 | 19p13 | MIMAT0000084 | ||
| hsa_miR_30e_5p | 0.527 | 1p34 | MIMAT0000692 | ||
| hsa_miR_16 | 0.540 | 13q14 | MIMAT0000069 | ||
| hsa_miR_142_5p | 0.541 | 17q22 | MIMAT0000433 | ||
| MDA-MB-435 | DOWN | hsa_miR_34a | 102.208 | 1p36 | MIMAT0000255 |
| hsa_miR_126 | 99.692 | 9q34 | MIMAT0000445 | ||
| hsa_miR_224 | 53.970 | Xq28 | MIMAT0000281 | ||
| hsa_let_7b | 51.088 | 22q13 | MIMAT0000063 | ||
| hsa_miR_155 | 50.785 | 21q21 | MIMAT0000646 | ||
| hsa_miR_139 | 31.486 | 11q13 | MIMAT0000250 | ||
| hsa_miR_199a | 28.600 | 19p13 | MIMAT0000231 | ||
| hsa_miR_452 | 25.500 | Xq28 | MIMAT0001635 | ||
| hsa_miR_148a | 24.778 | 7p15 | MIMAT0000243 | ||
| hsa_miR_200b | 20.446 | 1p36 | MIMAT0000318 | ||
| hsa_miR_222 | 16.220 | Xp11 | MIMAT0000279 | ||
| hsa_miR_200c | 12.161 | 12p13 | MIMAT0000617 | ||
| hsa_miR_380_3p | 11.759 | 14q32 | MIMAT0000735 | ||
| hsa_miR_503 | 7.627 | Xq26 | MIMAT0002874 | ||
| hsa_miR_138 | 7.141 | 3p21 | MIMAT0000430 | ||
| hsa_miR_485_5p | 6.773 | 14q32 | MIMAT0002175 | ||
| hsa_let_7f | 6.644 | 9q22 | MIMAT0000067 | ||
| hsa_miR_21 | 6.037 | 17q22 | MIMAT0000076 | ||
| hsa_miR_152 | 5.913 | 17q21 | MIMAT0000438 | ||
| hsa_miR_125b | 5.000 | 11q24 | MIMAT0000423 | ||
| hsa_miR_100 | 4.076 | 11q24 | MIMAT0000098 | ||
| hsa_miR_210 | 4.040 | 11p15 | MIMAT0000267 | ||
| hsa_miR_520h | 4.031 | 19q13 | MIMAT0002867 | ||
| hsa_miR_221 | 3.980 | Xp11 | MIMAT0000278 | ||
| MDA-MB-435 | UP | hsa_miR_146a | 0.001 | 5q33 | MIMAT0000449 |
| hsa_miR_363 | 0.001 | Xq26 | MIMAT0000707 | ||
| hsa_miR_506 | 0.002 | Xq27 | MIMAT0002878 | ||
| hsa_miR_508 | 0.003 | Xq27 | MIMAT0002880 | ||
| hsa_miR_509 | 0.003 | Xq27 | MIMAT0002881 | ||
| hsa_miR_513 | 0.003 | Xq27 | MIMAT0002877 | ||
| hsa_miR_31 | 0.004 | 9p21 | MIMAT0000089 | ||
| hsa_miR_105 | 0.006 | Xq25 | MIMAT0000102 | ||
| hsa_miR_211 | 0.009 | 15q13 | MIMAT0000268 | ||
| hsa_miR_510 | 0.013 | Xq27 | MIMAT0002882 | ||
| hsa_miR_20b | 0.016 | Xq26 | MIMAT0001413 | ||
| hsa_miR_146b | 0.025 | 10q24 | MIMAT0002809 | ||
| hsa_miR_9_AS | 0.028 | 1q22 | MIMAT0000442 | ||
| hsa_miR_204 | 0.032 | 9q21 | MIMAT0000265 | ||
| hsa_miR_501 | 0.034 | Xp11 | MIMAT0002872 | ||
| hsa_miR_500 | 0.038 | Xp11 | MIMAT0002871 | ||
| hsa_miR_502 | 0.042 | Xp11 | MIMAT0002873 | ||
| hsa_miR_362 | 0.045 | Xp11 | MIMAT0000705 | ||
| hsa_miR_188 | 0.061 | Xp11 | MIMAT0000457 | ||
| hsa_miR_185 | 0.070 | 22q11 | MIMAT0000455 | ||
| hsa_miR_497 | 0.079 | 17p13 | MIMAT0002820 | ||
| hsa_miR_196a | 0.087 | 17q21 | MIMAT0000226 | ||
| hsa_miR_195 | 0.089 | 17p13 | MIMAT0000461 | ||
| hsa_miR_96 | 0.182 | 7q32 | MIMAT0000095 |
Small RNAs were isolated from cell lines not expressing (MDA-MB-231 or MDA-MB-435) or expressing BRMS1, labeled with Cy5 or Cy3 and hybridized to arrays containing 328 human microRNA. Arrays were performed 3 times in independent experiments. Only consistent changes are shown in this table. Sanger database v12: http://microrna.sanger.ac.uk/sequences/. Subsequent to the evaluation of miR expression on the microarrays, homologs of some miR have been submitted to the Sanger databases. The chromosomal locations provided in this table correspond to the first miR deposited into the database.