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. Author manuscript; available in PMC: 2010 Mar 4.
Published in final edited form as: Cell. 2009 Sep 4;138(5):947–960. doi: 10.1016/j.cell.2009.06.033

Figure 4. NMJ boutons of flower mutants exhibit typical endocytic phenotypes at the EM level.

Figure 4

(A-H) TEM of type Ib boutons of NMJs in A2 or A3 segments in wild-type controls (A, C, and E) and mutants (B, D, F, G, and H) at rest (A, B, E, and F) or stimulated (C, D, G, and H). Mutant boutons exhibit a significant depletion in the number of SVs (compare A and B; compare E and F). Most of the residual SVs are associated with active zones in mutants (B and F). Mutant terminals display omega structures (G) and collared pits (H) which are rarely observed in wild-type control boutons. (I-K) Quantification of the number of SVs (I), the size of SVs (J), and SV intermediates (K). Type Ib boutons of NMJs in third instar wild-type controls (n=20), DB25/DB25 (n=23), DB25/DB56 (n=20), wild-type controls (Stimulated) (n=21), and DB25/DB56 (Stimulated) (n=26) were analyzed. At least three animals for each genotype were used. P value: *, P<0.05; **, P<0.01; ***, P<0.001. Error bars indicate SEM. Scale bar: 200 nm in A-F; 20 nm in G and H.