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. Author manuscript; available in PMC: 2010 Mar 4.
Published in final edited form as: Cell. 2009 Sep 4;138(5):947–960. doi: 10.1016/j.cell.2009.06.033

Figure 6. Heterologous expression of Flower allows Ca2+ influx.

Figure 6

(A-C) Images of Drosophila SGs captured over 1 hr period. SGs derived from third instar larvae expressing wild-type Flower-PB or Flower-PB with an E79Q substitution were loaded with 4 μM Fluo 4-AM and 100 μM [Ca2+]o. (D) Quantification of the Fluo 4-AM fluorescence level in A-C. (E) Similar as D but incubation in 10 μM [Ca2+]o. (F) SGs coexpressing Flower-PB and GCaMP1.6 or lacZ (as a control transgene) and GCaMP1.6 were bathed sequentially in various [Ca2+]o (0.1 mM, 1 mM, and 0 mM plus 10 mM EGTA). GCaMP1.6 fluorescence in 0 mM plus 10 mM EGTA at 90 min was selected as F0. Quantification of ΔF/F0 of GCaMP1.6 is shown. The number of SGs analyzed is indicated. Scale bars: 50 μm in A-C. Error bars indicate SEM.