Table 1.
Primers used for RT-PCR
| Gene name | Sequence (upstream and downstream, respectively) | Reference |
|---|---|---|
| Ap2 | TCCGACCCGAACGAGCAAGTA | |
| ATCAGGACCGGGCAATGTTCTA | This work | |
| Bmp4 | GATCTCAGACTCAACGGCAC GCATGTACGGATAAGTCGATC | De Robertis* |
| Chordin | CCTCCAATCCAAGACTCCAGAG | |
| GGAGGAGGAGGAGCTTTGGGACAAG | De Robertis* | |
| Cerberus | GCTGAACTATTTGATTTCACC | |
| ATGGCTTGTATTCTGTGGGGCG | De Robertis* | |
| Fgf3 | GTCATTTGTTTCCAGACTTC | |
| TATCTGTAGGTGGTACTTAG | This work | |
| Fgf4 | TTACCGGACGGAAGGATA | |
| CCTCGATTCGTAAGCGTT | This work | |
| Fgf8 | CTGGTGACCGACCAACTAAG | |
| ACCAGCCTTCGTACTTGACA | This work | |
| Foxd3 | GGAGGGAGGGGGCAATGCAC | |
| CCCCGAGCTCGCCTACT | (Pohl and Knochel, 2001) | |
| Frzb-1 | GACCACTGAATGTAGCCAGGAC | |
| GGAGATGCAGACTCCTCTGTCA | De Robertis* | |
| Lrig3 | GTGCAGTGTACAAGGCCTCA | |
| TTGGAATGTACACGGACTGC | This work | |
| Lrig3A intron | CGGCTAGACTAGAGTGTGCT | |
| AGCATCTTCAAGGTCTGTGT | This work | |
| Lris3B intron | CAGCTAGATTAGAATGTGCAGCG | |
| CCATTAATGGCCGTAGAAACGATG | This work | |
| Msx1 | GCTAAAAATGGCTGCTAA | |
| AGGTGGGCTGTGTAAAGT | De Robertis* | |
| Odc | CAGCTAGCTGTGGTGTGG | |
| CAACATGGAAACTCACACC | De Robertis* | |
| Pax3 | CTACCTCGGTTTCTTGACTG | |
| TGGTCAATCCTTCTTAATGG | This work | |
| Siamois | CAGGTTTGGTTTCAGAACAG | |
| TTCAGGCCAGTTGAATGTAT | This work | |
| Slug | TCCCGCACTGAAAATGCCACGATC | |
| CCGTCCTAAAGATGAAGGGTATCCTG | (LaBonne and Bronner-Fraser, 1998) | |
| Snail | AAGCACAATGGACTCCTT | |
| CCAATAGTGATACACACC | (LaBonne and Bronner-Fraser, 1998) | |
| Sox2 | GAGGATGGACACTTATGCCCAC | |
| GGACATGCTGTAGGTAGGCGA | De Robertis* | |
| Sox3 | TGATGCAGGACCAGTTGGGC | |
| TGAAGTGAAGGGTCGCTGGC | (Ishibashi and Yasuda, 2001) | |
| Sox9 | AAGCAGAATGTCCTCTGTGA | |
| AAGGCCAGATTCAGTTCTTC | This work | |
| Sox17β | GTCATGGTAGGAGAGAAC | |
| ATCTGTTTAGCCATCACTG | De Robertis* | |
| VegT | CAAGTAAATGTGAGAAACCG | |
| CAA ATACACACACATTTCCC | De Robertis* | |
| Xbra | TTAAGTGCTGTAATCTCTTCA | |
| GCTGGAAGTATGTGATGGAG | De Robertis* | |
| Xlim-1 | GAAGGATGAGACCACTGGTGG | |
| CACTGCCGTTTCGTTCATTTC | De Robertis* | |
| XTwist | AGTCCGATCTCAGTGAAGCGCA | |
| TGTGTGTGGCCTGAGCTGTAG | (LaBonne and Bronner-Fraser, 1998) | |
| XVent-1 | TTCCCTTCAGCATGGTTCAAC | |
| GCATCTCCTTGGCATATTTGG | (Gawantka et al., 1995) | |
| Zic1 | ATGAAGGTCCACGAAGCATC | |
| CGTGCTGTGATTGGACGTGT | (Nakata et al., 1998) |