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. Author manuscript; available in PMC: 2009 Sep 23.
Published in final edited form as: Mol Genet Genomics. 2008 Apr 23;280(1):25–39. doi: 10.1007/s00438-008-0342-5

Fig. 10.

Fig. 10

Analysis of Aac1-Aac2 chimeras and the gain-of-function P89L and A96V mutants of Aac1p. (A) Serial dilutions of CS494-2B (aac2Δ, GAL10-AAC2, sal1-1) transformants expressing Aac11-183-Aac2193-318 and Aac21-192-Aac1184-309 from the AAC2 promoter were spotted onto YPGal (Galactose) and YPD (Glucose). (B) Serial dilutions of CS494-2B transformants expressing aac1P89L and aac1A96V from the AAC2 promoter were spotted onto YPGal and YPD. (C) aac1P89L and aac1A96V are partially respiratory deficient. CS341 (aac2Δ, but SAL1) cells expressing aac1P89L and aac1A96V were diluted in water and spotted on YPD and YPGly (Glycerol).