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. Author manuscript; available in PMC: 2009 Sep 23.
Published in final edited form as: Circ Res. 2008 May 22;102(12):1512–1519. doi: 10.1161/CIRCRESAHA.108.172064

Figure 3. Recruitment of src family kinases to platelet CD36 is essential for oxLDL-mediated signaling.

Figure 3

(A) Human platelets were incubated with the JNK inhibitor SP600125, src inhibitor AG1879, PKC inhibitor GO6983 or PI3K inhibitor LY294002 for 30 minutes prior to incubation with 50 μg/ml oxLDL. The platelet lysates were then analyzed by immunoblot as in figure 1 for JNK phosphorylation. (B) Platelets were incubated with oxLDL or native LDL and then lysed. CD36 was precipitated by FA6 anti-CD36 IgG. Precipitates were analyzed by immunoblot with antibodies to CD36, phospho-src (Y416), Fyn and Lyn. (C) Platelets were incubated with 50 μg/ml oxLDL or native LDL and then lysed. Lyn was immunoprecipitated from lysates and the precipitates were analyzed by immunoblot with a phosphotyrosine antibody (4G10) and Lyn antibody.