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PrP amyloid plaques in Tg(A116V) mice are composed of full-length PrPA116V. IHC was performed using four PrP antibodies that recognize four distinct regions of mature PrP23-230, as indicated. All plaques are from the granule cell layer of the cerebellar cortex. “No Primary Ab” signifies all of the IHC steps were performed except incubation of the slide with a primary anti-PrP antibody.
