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. 2009 Jul 30;28(18):2835–2845. doi: 10.1038/emboj.2009.209

Figure 5.

Figure 5

Binding affinities of Arfs–JIP4-LZII complexes studied by surface plasmon resonance (SPR). (A) Real-time association and dissociation SPR profiles corresponding to the injection of ARF6 (3.9 μM) over immobilized wild-type and variant JIP4-LZII and (B) ARF6/(JIP4)2 molar ratio as a function of ARF6 concentration and corresponding fitted Kd values. (C) Real-time association and dissociation SPR profiles corresponding to the injection of the different Arf variants (1.8 μM) over immobilized JIP4-LZIIWT and (D) ARF/(JIP4)2 molar ratio as a function of Arf concentration and corresponding fitted Kd values.