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. 2009 Jul 30;28(18):2733–2747. doi: 10.1038/emboj.2009.216

Figure 5.

Figure 5

CRTCs elicit alternative ED I splicing independent of core promoter context. (A) Effect of PKA and CRTCs on transcriptional activity of a TATA-less CRE-containing promoter. Transient transfection assays of CDC37 luciferase reporter co-transfected into HEK293T cells in the presence or absence of PKA or CRTC were analysed for luciferase activity (n=6 wells; mean±s.e.m.; asterisk denotes P-value ⩽0.05). (B) TaqMan real-time qPCR analysis of PKA and CRTCs effects on CDC37 ED I minigene exon skipping. The relative exon skipping levels are expressed as the ratio of skipped versus included transcripts (n=3 experiments; mean±s.e.m.; asterisk denotes P-value ⩽0.05). (C) TaqMan real-time qPCR analysis of PKA and CRTCs affects on CDC37 ED I minigene reporter gene expression. Relative amounts of ED I reporter transcripts obtained by each transfection were plotted as folds relative to basal transcription (n=3 experiments; mean±s.e.m.; asterisk denotes P-value ⩽0.05).