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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: J Am Soc Mass Spectrom. 2009 Apr 16;20(8):1504–1513. doi: 10.1016/j.jasms.2009.04.004

Fig. 2.

Fig. 2

ESI MS/MS analysis of the 18O labeled cross-linked peptide with mass of 2466 Da reconstituted from the triply charged ion of m/z 823.1. The sequence of the peptide was assigned with single letter abbreviation based on the fragment ions observed for the cross-linked peptide segments. N-terminal b ions and C-terminal y ions resulting from the amide bond cleavage are labeled. The MS/MS spectrum indicates that K30 of the peptide segment Y[26-39]]K (designated as α) linked to K389 of D[387-391]R (designated as β), via C8H10O2, the cross-linking bridge from DSS, with C-terminal of K39 and R391 labeled with 18O. 18O labeling facilitated the assignment of the fragment ions. The m/z values for the fragment ions are labeled (the m/z values obtained from 16O labeling are listed in the parentheses). Inset shows the charged state for the indicated ions.