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. Author manuscript; available in PMC: 2009 Sep 23.
Published in final edited form as: Nat Protoc. 2009;4(3):372–384. doi: 10.1038/nprot.2009.3
Reagent for PCR Volume (μl) for each (25 μl sample) Final concentration (with template)
Taq DNA Polymerase (added last) 0.25 50 U ml−1
10× buffer 2.5
dNTPs (10 mM each, combined) 0.5 200 μM
Forward primer: VH3a and VH3b or PanVκ 0.5 1.2 μM
Reverse primer: PW-Cgamma or CK494-516 0.5 1.2 μM
dH2O (nuclease free) 17.25–19.25 (to 24 μl total volume)
Template 1.0