Reagent for PCR | Volume (μl) for each (25 μl sample) | Final concentration (with template) |
---|---|---|
Taq DNA Polymerase (added last) | 0.25 | 50 U ml−1 |
10× buffer | 2.5 | 1× |
dNTPs (10 mM each, combined) | 0.5 | 200 μM |
Forward primer: VH3a and VH3b or PanVκ | 0.5 | 1.2 μM |
Reverse primer: PW-Cgamma or CK494-516 | 0.5 | 1.2 μM |
dH2O (nuclease free) | 17.25–19.25 (to 24 μl total volume) | — |
Template | 1.0 | — |