Skip to main content
. 2009 Jul 31;10(9):1036–1042. doi: 10.1038/embor.2009.139

Figure 1.

Figure 1

ARF has a role in nucleotide-excision repair that is independent of p53. The nucleotide-excision-repair activity following ultraviolet irradiation was measured in WT, ARF−/−, p53−/−MDM2−/− (DKO) and p53−/−MDM2−/−ARF−/− (TKO) MEFs by UDS (Smith et al, 2000). UDS was quantified by counting the number of 3H-thymidine grains per nucleus in 30 nuclei per cell type. Autoradiographs of the grains in nuclei are shown in the upper panel, and grains per nucleus and means±s.e.m. are shown in the lower panel. TKO compared with DKO P=0.0058 and WT compared with ARF−/− P<0.0001. ARF, alternative reading frame; DKO, double knockout; MDM2, murine double minute 2; MEF, mouse embryonic fibroblast; TKO, triple knockout; UDS, unscheduled DNA synthesis; WT, wild type.