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. 2009 Aug 14;10(9):1015–1021. doi: 10.1038/embor.2009.152

Figure 1.

Figure 1

Cytological characterization of centromeric heterochromatin in sperm and vegetative nuclei of wild-type (Col-0) mature pollen. (A) A DAPI-stained mature pollen grain. (B) FISH image of SN and VN of pollen with a probe for 180CEN repeats. Approximately 1,000 vegetative nuclei were examined and all of them showed extremely dispersed FISH signals. Immunostaining of (C) bulk histone H3; (D) CenH3 (HTR12) and H3K9me2; and (E) H3K9me2 and H3K27me1 in SN and VN. Immunostaining patterns similar to those shown were observed in all approximately 100 nuclei examined. (B–E) Nuclei were counterstained with DAPI (blue). Scale bars, 3 μm. DAPI, 4',6-diamidino-2-phenylindole; FISH, fluorescence in situ hybridization; SN, sperm nuclei; VN, vegetative nuclei.