Figure 10.

MPEP, an mGluR antagonist, does not rescue the long sleeping phenotype due to loss of dFMR1. Age-matched flies, harvested within 24 h of eclosure, were tested on cornmeal molasses media for 2 d. On the third day (at lights on), flies were transferred to tubes with sucrose media containing vehicle or 86 μm MPEP, tested for 6 d, and then transferred to fresh sucrose media and monitored for seven additional days. A, Daily sleep amount (min/24 h) before, during, and after MPEP treatment. B, Waking activity (number of beam crossings/min) before, during, and after MPEP treatment. Solid circles indicated significant difference between MPEP-treated Δ50M amorphs and MPEP-treated EP3517 hypomorphs; triangles represent significant difference between untreated Δ50M amorphs and untreated EP3517 hypomorphs (p < 0.05, Mann–Whitney test). Number of flies were as follows: MPEP-treated EP3517 = 48, MPEP-treated Δ50M = 39, untreated EP3517 = 41, untreated Δ50M = 32.