Figure 3.

Intracellular leucine concentration in the liver lobes of the dogs within the three experimental groups. At the end of the infusion period, liver samples were quickly removed and frozen in liquid nitrogen and stored at −80°C until analyzed. Liver samples were prepared as described in “Materials and Methods” and leucine was determined as described in Donahue et al. (19). The results represent the mean ± SE of 9 animals for each group. The statistical evaluation of the data was performed by a 2-way repeated-measures analysis of variance to test the group and lobe effects and lobe × group interaction. When significant, the Bonferroni test was employed for post hoc analysis. Differences were considered significant when P < 0.05.

Group effect P<0.0001, Lobe effect P<0.036 and no significant Lobe × Group interaction. EuAA significantly different from PoAA and PeAA.; PeAA not significantly different from PoAA.