Figure 5.

Hepatic and albumin fractional protein synthesis rates. Amino acids were infused as described in the legend to Figure 1 and Table 1. Arterial, portal and hepatic blood samples were drawn at times indicated in the figure. At the end of the infusion period, liver samples were quickly removed and frozen in liquid nitrogen and stored at −80°C until analyzed. Liver samples were prepared as described in “Materials and Methods” and leucine and leucine SA were determined as described in Donahue et al. (19). Liver protein synthesis was analyzed on the seven hepatic lobes in each dog. Albumin synthesis was determined kinetically from 210 to 300 min. The results represent the mean ± SE of 9 animals for each group.

For liver synthesis, the statistical evaluation of the data was performed by a 2-way measures analysis of variance to test the group and lobe effects and lobe × group interaction. When significant, the Bonferroni test was employed for post hoc analysis. Differences were considered significant when P < 0.05. Group effect P<0.0001; Lobe effect P<0.025 and no significant interaction interaction. PoAA significantly different from EuAA and PeAA. PeAA not significantly different to EuAA

For albumin synthesis, the statistical evaluation of the data was performed by a 2-way repeated-measures analysis of variance to test the group and time effects and time × group interaction. When significant, the Bonferroni test was employed for post hoc analysis. Differences were considered significant when P < 0.05. No significant time effect; Group effect P<0.0001 and no significant interaction. PoAA significantly different from EuAA and PeAA. PeAA not significantly different from EuAA