Table 2.

Hepatic plasma flows, hepatic sinusoidal insulin and glucagon concentrations

	Basal		Amino acid infusion
Time (min)	90	120	150	210	240	270	300	Variance analysis
Arterial flows (ml/kg/min)
EuAA	3.1±0.2	3.0±0.2	4.0±0.3	4.3±0.4	4.1±0.4	4.5±0.4	4.5±0.4	Group effect = NS
PeAA	3.1±0.2	3.1±0.2	3.9±0.3	4.2±0.2	3.8±0.3	4.1±0.2	4.3±0.3	Time effect P<0.001
PoAA	3.0±0.3	3.2±0.3	3.7±0.5	4.5±0.4	4.1±0.4	4.0±0.4	4.1±0.3	Time×Group =NS
Portal flows (ml/kg/min)
EuAA	14.6±1.0	14.9±1.0	13.0±0.7	12.5±0.6	12.6±0.8	13.1±1.0	13.1±0.9	Group effect = NS
PeAA	13.6±0.8	14.0±0.9	12.4±0.9	12.4±0.8	12.6±0.8	12.9±0.8	12.9±0.9	Time effect P<0.006
PoAA	13.7±0.6	14.2±0.6	12.7±0.8	12.4±0.7	12.7±0.6	12.8±0.6	12.9±0.7	Time×Group =NS
Hepatic sinusoidal insulin (mU/ml)
EuAA	17.1±1.6	15.4±2.4	75.8±9.6	74.7±5.7	60.1±8.8	63.9±9.2	74.9±10.7	Group effect = NS
PeAA	16.1±2.1	20.0±5.3	63.5±4.8	72.1±11.2	70.5±5.7	73.4±4.2	82.7±4.4	Time effect P<0.0001
PoAA	16.8±3.1	20.7±3.8	88.2±18.4	74±8.1	69.8±7.3	83.7±6.3	78.2±9.2	Time×Group =NS
Hepatic sinusoidal glucagon (pg/ml)
EuAA	53.2± 4.7	49.6± 9.0	144.6±16.0	138.7±16.5	134.0±11.3	142.7±13.7	124.0±16.2	Group effect = NS
PeAA	42.8±6.9	41.6±6.2	110.3±16.5	112.2±5.3	113.0±5.4	103.1±4.3	107.7±7.7	Time effect P<0.001
PoAA	30.7±4.2	32.4±3.8	122.3±15.7	90.6±7.8	96.5±10.5	98.2±7.8	106.6±13.6	Time×Group =NS

Data are expressed as means ± SE (n=9 in each group. The statistical evaluation of the data was performed by a 2-way repeated-measures analysis of variance to test the group and time effects and time × group interaction. When significant, the Bonferonni test was employed for post hoc analysis. Differences were considered significant when P < 0.05. NS= non significant