Table 3.
Arterial and portal amino acid concentrations, hepatic aminoacid load and amino acid arterio-portal gradient in the three experimental groups EuAA, PeAA and PoAA
| Arterial concentration µM |
Portal concentration µM |
Hepatic load (µmol/kg/min) |
ArterioPortal gradient µM |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| AA infusion | AA infusion | Basal | AA infusion | AA infusion | |||||||
| Mean | SE | Mean | SE | Mean | SE | Mean | SE | Mean | SE | ||
| ALA | EuAA | 459.2 | 42.0 a | 542.8 | 44.7 a | 7.0 | 0.8 a | 9.1 | 1.0 a | −83.7 | 8.2 a |
| PeAA | 539.6 | 57.3 b | 601.9 | 95.5 b | 6.0 | 0.6 a | 9.6 | 1.0 a | −62.3 | 13.8 a | |
| PoAA | 470.2 | 40.4 a | 777.4 | 101.6 c | 7.8 | 1.0 a | 11.0 | 1.1 a | −307.2 | 19.4 b | |
| ARG | EuAA | 157.8 | 13.5 ab | 146.8 | 13.6 a | 2.4 | 0.2 a | 2.6 | 0.3 a | 10.9 | 3.0 a |
| PeAA | 183.7 | 19.0 a | 169.6 | 26.9 a | 1.5 | 0.2 b | 2.8 | 0.3 a | 14.1 | 5.0 a | |
| PoAA | 121.9 | 8.1 b | 233.7 | 35.4 b | 1.8 | 0.1 ab | 2.9 | 0.3 a | −111.9 | 17.5 b | |
| ASN | EuAA | 78.2 | 6.5 a | 70.1 | 6.7 a | 1.3 | 0.1 a | 1.3 | 0.2 a | 8.1 | 3.4 a |
| PeAA | 107.7 | 10.2 b | 101.8 | 15.3 b | 1.2 | 0.1 a | 1.7 | 0.2 b | 5.9 | 2.4 a | |
| PoAA | 60.4 | 4.7 a | 128.3 | 18.7 b | 1.3 | 0.1 a | 1.7 | 0.2 b | −67.9 | 7.7 b | |
| ASP | EuAA | 7.3 | 1.8 a | 8.7 | 1.9 a | 0.2 | 0.0 a | 0.1 | 0.0 a | −1.3 | 0.8 a |
| PeAA | 17.7 | 2.3 b | 17.9 | 2.3 b | 0.2 | 0.0 a | 0.3 | 0.0 b | −0.2 | 1.2 a | |
| PoAA | 12.2 | 1.5 ab | 21.2 | 2.9 b | 0.2 | 0.0 a | 0.3 | 0.0 b | −9.0 | 1.6 b | |
| CYS | EuAA | 36.6 | 4.1 a | 37.2 | 3.2 a | 0.5 | 0.0 a | 0.6 | 0.1 a | −0.7 | 1.7 a |
| PeAA | 55.7 | 6.2 b | 53.1 | 7.7 b | 0.6 | 0.1 a | 0.9 | 0.1 a | 2.5 | 1.6 a | |
| PoAA | 24.1 | 5.1 a | 54.3 | 9.4 b | 0.4 | 0.1 a | 0.7 | 0.1 a | −30.2 | 5.7 b | |
| GLN | EuAA | 754.1 | 32.1 a | 648.3 | 28.7 a | 13.4 | 1.2 a | 12.7 | 1.0 a | 105.7 | 8.3 a |
| PeAA | 1005.6 | 67.6 b | 934.4 | 111.6 b | 15.8 | 1.3 a | 16.4 | 0.6 ab | 62.4 | 19.3 a | |
| PoAA | 781.3 | 25.5 a | 989.7 | 125.2 b | 17.5 | 1.7 a | 19.1 | 2.2 b | −208.4 | 32.6 b | |
| GLU | EuAA | 74.7 | 4.2 a | 80.8 | 4.5 a | 0.7 | 0.1 a | 0.9 | 0.1 a | −6.0 | 1.6 a |
| PeAA | 131.4 | 13.8 b | 133.4 | 16.4 b | 0.7 | 0.1 a | 1.4 | 0.2 b | −2.1 | 3.5 a | |
| PoAA | 79.4 | 5.8 a | 132.5 | 17.5 b | 0.8 | 0.1 a | 1.5 | 0.1 b | −53.1 | 8.6 b | |
| GLY | EuAA | 319.3 | 34.9 a | 266.6 | 32.0 a | 4.6 | 0.4 a | 4.7 | 0.5 a | 52.7 | 13.8 a |
| PeAA | 313.6 | 21.6 a | 301.5 | 41.5 b | 4.1 | 0.4 a | 5.0 | 0.5 a | 12.2 | 11.4 b | |
| PoAA | 206.2 | 16.8 b | 317.4 | 47.2 b | 4.6 | 0.4 a | 4.6 | 0.5 a | −111.2 | 22.3 c | |
| HIS | EuAA | 57.3 | 3.2 a | 56.2 | 3.4 a | 1.0 | 0.1 a | 1.0 | 0.1 a | 1.1 | 1.6 a |
| PeAA | 87.9 | 13.2 b | 86.2 | 11.0 b | 1.0 | 0.1 a | 1.4 | 0.2 b | 1.7 | 1.8 a | |
| PoAA | 59.4 | 6.1 a | 87.8 | 12.9 b | 0.9 | 0.1 a | 1.3 | 0.1 b | −28.4 | 4.1 b | |
| ISO | EuAA | 63.3 | 6.6 a | 56.1 | 5.7 a | 1.1 | 0.1 a | 1.0 | 0.1 a | 7.2 | 2.4 a |
| PeAA | 169.5 | 24.2 b | 152.8 | 28.1 b | 1.3 | 0.2 a | 2.6 | 0.4 b | 16.7 | 5.5 b | |
| PoAA | 89.4 | 6.3 a | 177.1 | 24.2 b | 1.3 | 0.1 a | 2.3 | 0.2 b | −87.8 | 8.0 c | |
| LEU | EuAA | 232.6 | 14.7 a | 219.0 | 16.3 a | 3.4 | 0.3 a | 3.8 | 0.3 a | 13.5 | 6.7 a |
| PeAA | 412.3 | 45.8 b | 392.1 | 65.2 b | 3.0 | 0.3 a | 6.5 | 0.8 b | 20.2 | 8.0 a | |
| PoAA | 211.5 | 19.1 a | 382.2 | 55.8 b | 2.7 | 0.3 a | 5.2 | 0.6 b | −170.6 | 17.1 b | |
| LYS | EuAA | 214.2 | 14.7 a | 199.5 | 20.0 a | 2.7 | 0.3 a | 2.4 | 0.3 a | 9.8 | 4.9 a |
| PeAA | 364.9 | 26.4 b | 361.5 | 46.0 b | 2.8 | 0.2 a | 5.2 | 0.3 b | 3.0 | 11.3 a | |
| PoAA | 231.0 | 19.2 a | 390.6 | 50.3 b | 2.6 | 0.6 a | 5.4 | 0.5 b | −159.6 | 20.6 b | |
| MET | EuAA | 55.6 | 2.9 a | 50.5 | 2.8 a | 0.8 | 0.1 a | 0.9 | 0.1 a | 5.1 | 2.0 a |
| PeAA | 99.9 | 14.5 b | 93.9 | 18.0 b | 0.9 | 0.1 a | 1.5 | 0.2 b | 5.9 | 3.2 a | |
| PoAA | 44.4 | 4.5 a | 87.8 | 13.2 b | 0.9 | 0.1 a | 1.5 | 0.2 b | −43.5 | 4.7 b | |
| PHE | EuAA | 50.0 | 2.8 a | 45.1 | 2.7 a | 0.9 | 0.1 a | 0.8 | 0.1 a | 4.9 | 2.1 a |
| PeAA | 109.4 | 6.9 b | 107.5 | 14.6 b | 0.9 | 0.1 a | 1.8 | 0.1 b | 1.9 | 2.4 a | |
| PoAA | 49.2 | 5.3 a | 112.6 | 15.8 b | 0.9 | 0.1 a | 1.5 | 0.2 b | −63.3 | 7.5 b | |
| PRO | EuAA | 120.5 | 9.0 a | 126.6 | 7.8 a | 2.0 | 0.3 a | 2.2 | 0.4 a | −3.1 | 2.6 a |
| PeAA | 357.3 | 32.6 b | 362.5 | 57.6 b | 2.5 | 0.2 a | 5.9 | 0.6 b | −5.2 | 8.9 a | |
| PoAA | 183.0 | 12.0 c | 350.5 | 49.6 b | 2.8 | 0.3 a | 4.7 | 0.5 b | −167.5 | 15.8 b | |
| SER | EuAA | 126.5 | 7.2 a | 113.8 | 6.1 a | 2.0 | 0.2 a | 2.0 | 0.2 a | 12.7 | 2.8 a |
| PeAA | 233.8 | 33.7 b | 210.6 | 36.0 b | 2.0 | 0.3 a | 3.6 | 0.5 b | 23.2 | 5.2 b | |
| PoAA | 114.2 | 7.4 a | 258.1 | 39.0 b | 2.1 | 0.2 a | 3.3 | 0.3 b | −143.9 | 17.9 c | |
| THR | EuAA | 199.0 | 13.7 a | 233.3 | 15.6 a | 3.9 | 0.4 a | 3.9 | 0.4 a | −20.1 | 4.4 a |
| PeAA | 346.3 | 47.0 b | 355.8 | 57.2 b | 2.9 | 0.3 a | 5.9 | 0.9 b | −9.5 | 9.0 b | |
| PoAA | 258.2 | 22.3 a | 380.8 | 50.1 b | 3.6 | 0.6 a | 5.4 | 0.7 b | −122.6 | 15.4 c | |
| TRP | EuAA | 51.5 | 5.8 a | 46.2 | 5.0 a | 0.6 | 0.1 a | 0.5 | 0.1 a | 3.2 | 2.0 a |
| PeAA | 120.0 | 17.1 b | 118.2 | 20.0 b | 1.0 | 0.1 a | 2.0 | 0.3 b | 1.8 | 2.3 a | |
| PoAA | 146.5 | 14.7 b | 185.3 | 30.3 c | 1.2 | 0.1 a | 2.7 | 0.3 c | −38.8 | 8.5 b | |
| TYR | EuAA | 53.8 | 4.6 a | 48.7 | 4.4 a | 0.9 | 0.1 a | 0.9 | 0.1 ab | 5.1 | 2.7 a |
| PeAA | 42.1 | 4.6 a | 35.3 | 4.9 a | 0.7 | 0.1 a | 0.6 | 0.1 a | 6.8 | 1.1 a | |
| PoAA | 30.8 | 2.9 b | 66.9 | 10.0 b | 0.8 | 0.1 a | 0.9 | 0.1 b | −36.0 | 4.0 b | |
| VAL | EuAA | 217.0 | 10.8 a | 202.5 | 9.2 a | 4.1 | 0.2 a | 3.6 | 0.3 a | 14.5 | 4.0 a |
| PeAA | 465.9 | 24.2 b | 442.8 | 54.7 b | 3.7 | 0.3 a | 7.3 | 0.3 b | 23.0 | 6.9 a | |
| PoAA | 309.4 | 23.1 c | 462.4 | 65.2 b | 3.5 | 0.4 a | 6.6 | 0.6 b | −153.0 | 14.7 b | |
Data are expressed as means ± SE (n=9) in each group. The basal data are the mean of the values at 90 and 120 min. The AA infusion data are the mean of the determinations between 150 and 300 min. The statistical evaluation of the data was performed by a one way measures analysis of variance. Bonferroni adjusted data are shown. Differences were considered significant when P < 0.05. Values with different letters are significantly different within the same column, P <0.05.