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. Author manuscript; available in PMC: 2010 Apr 15.
Published in final edited form as: J Am Chem Soc. 2009 Apr 15;131(14):5257–5263. doi: 10.1021/ja809419f

Figure 1.

Figure 1

A selection cycle. Color coding: T7-promoter (17 nt) - dark red, 5'-primer (19 nt) - red, random region (50 nt) - black, HDV ribozyme (87 nt) - blue. [α 32P G] pool RNA has 69 nt; HDV ribozyme (67 nt) is distinct because it can be 3′-elongated to 87 nt without change in reactivity. Self-cleavage results in 2′,3′-cyclic phosphate at the 3′-end of transcript RNA, that must be opened and dephosphorylated.