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. 2009 Jul 8;58(10):2355–2364. doi: 10.2337/db09-0432

FIG. 2.

FIG. 2.

Effect of LY333531 (LY)on PKCβ2 phosphorylation and iNOS expression in control and diabetic heart tissues. A: Representative Western blot showing phospho-PKCβ2 (Thr641) in comparison with total PKCβ2 expression levels in ventricular tissues of control (C), diabetic (D), control LY333531-treated (C-LY), and diabetic LY333531-treated (D-LY) rats. The densitometric values of phospho-PKCβ2 were normalized to corresponding total PKCβ2 expression levels and the relative band intensities are expressed as means ± SE (n = 5). *P < 0.05 compared with C, C-LY, and D-LY groups. B: Representative Western blot showing iNOS expression in the ventricular tissues of C, D, C-LY, and D-LY groups. iNOS protein was immunoprecipitated from heart ventricular tissue lysates using mouse monoclonal anti-iNOS antibody. Equal amounts of immunoprecipitated complex were loaded on to the gels and were subjected to SDS-PAGE. Densitometric data are expressed as percentage of control. All values are expressed as means ± SE (n = 4). *P < 0.05 compared with C, C-LY, and D-LY groups.