FIG. 6.

Fatty acid oxidation, lipogenesis, and glucose uptake in quadriceps muscle in naringenin-treated mice. Ldlr^−/− mice (n = 12/group) were fed a standard diet or a high-fat diet alone or supplemented with naringenin (1 or 3%) for 4 weeks. A and B: Triglyceride and cholesteryl ester concentrations in muscle lipid extracts. C and D: Expression of Pgc1α (C) and Cpt1β (D) relative to Gapdh in muscle by qRT-PCR. E: Fatty acid oxidation in muscle (n = 6/group) as determined as [³H]palmitate conversion to ³H₂O. F and G: Expression of Ucp1 (F) and Ucp3 (G) relative to Gapdh in muscle by qRT-PCR. H: Synthesis of fatty acid in muscle (n = 6–8/group) obtained 60 min postinjection intraperitoneally with [¹⁴C]acetic acid. I: Expression of Srebp1c relative to Gapdh in muscle by qRT-PCR. J: Deoxyglucose uptake into muscle (n = 4–6/group) obtained 30 min postinjection intraperitoneally with [³H]deoxyglucose. Values are means ± SE, different letters are statistically different, P < 0.05. □, standard diet; ■, Western diet; ▨, Western diet and 1% naringenin; ▩, Western diet and 3% naringenin.