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. 2009 Jul 6;58(10):2303–2315. doi: 10.2337/db07-1781

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© 2009 by the American Diabetes Association.

Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details.

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FIG. 1. — Dissection of the mitochondrial web in primary β-cells using PAGFPmt. A: Projection of confocal images of a cell stained with TMRE. Note that mitochondria are densely packed in β-cells. B: Sequential 2-photon laser photoactivation of individual mitochondria. C: Summary of mitochondrial size distribution performed by 90 photoactivation steps in 9 cells. D: Tagging and tracking individual mitochondria in primary β-cells reveals fusion events (transfer of activated PAGFPmt to juxtaposed unlabeled mitochondria) that are then followed by fission events (separation of previously connected mitochondria). (A high-quality digital representation of this figure is available in the online issue.)