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. 2009 Oct 5;4(10):e7346. doi: 10.1371/journal.pone.0007346

Figure 2. Syndecan-1 and FGF-2 co-immunoprecipitate in the nucleus of the STAV-AB cells.

Figure 2

Co-IP and immunoblotting were performed on nuclear extracts of sub-confluent cultures of the STAV-AB cells, stably transfected with syndecan-1/EGFP construct or EGFP vector, as described in “Materials and Methods”. Nuclear extract (100 µg) was incubated with 3 µg of syndecan-1 antibody, or with no antibody (negative control). The nuclear precipitates from equal amounts of control and sample proteins were slot-blotted onto a nitrocellulose membrane and then probed with antibody to FGF-2. A crude nuclear extract from syndecan-1/EGFP transfected cells was used as positive control. Co-IP using a specific antibody to syndecan-1 also pulled down FGF-2, as compared to the negative control. The amount of FGF-2 was higher in nuclear extracts from syndecan-1 overexpressing cells, compared to the EGFP control.