Figure 3. Effects of drugs interfering with microtubule assembly and cell cycle progression.

The sub-cellular localizations of FGF-2 (A), syndecan-1 (B) and FGFR-1 (C) in the STAV-AB cells were detected after depolymerization of tubulin by vinblastine shown as co-localized paracrystalline structures (yellow crystals). Double staining was performed with tubulin (green) and either FGF-2 (red) (A) or syndecan-1 (red) (B), and both revealed strict co-localization of these components (yellow crystals, A, B). No co-localization of FGFR-1 (green) and tubulin (red) is detected (C), indicating a different transport route. Untreated STAV-FCS mesothelioma cells showed strong nuclear reactivity for syndecan-1 (D). Doxorubicin almost completely inhibited the nuclear transport of syndecan-1 in these cells (E).