Table 1. Primary antibodies used.
No. | Antigen | Clone | Dilution | Company/catalogue no. |
1 | Syndecan-1(CD138), mouse monoclonal IgG1 | B-B4 | 1∶4 | Serotec MCA681 H |
2 | Syndecan-1, goat polyclonal | - | 1∶20 | Santa Cruz sc-7099 |
3 | FGF-2, mouse monoclonal IgG2a | MC-GF1 | 1∶4 | Serotec MCA1400G |
4 | FGF-2, goat polyclonal | N-19 | 1∶20 | Santa Cruz sc-1390 |
5 | FGFR-1, mouse monoclonal IgM | VBS1 | 1∶20 | Biogenesis |
6 | HS, mouse monoclonal IgM | 10E4 | 1∶200 | Seikagaku 370255 |
7 | Heparanase, mouse monoclonal | mAB 130 | 1∶400 | InSight Ltd. |
8 | Heparanase, rabbit polyclonal | pAB 733 | 1∶10 | Vlodavsky et. al. |
9 | α-tubulin, mouse monoclonal IgG1 | B-5-1-2 | 1∶2000 | Sigma T5168 |