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. 2009 Oct 5;4(10):e7295. doi: 10.1371/journal.pone.0007295

Figure 4. Effect of HA-disabling mutations on growth characteristics of TBEV in mammalian models.

Figure 4

(A) Confluent monolayers of PS cells were infected with IC-D67G, IC-E122G, IC-D277A or pGGVsH virus at an estimated moi of 1 PFU/cell and virus yield in cell culture medium was determined at different time points post-infection by plaque assay. (B) Plaque morphology of specified Yar viruses and engineered mutants in PS cell monolayers. (C) Adult mice were inoculated intraperitoneally with 2000 PFU of IC-D67G, IC-E122G, IC-D277A or pGGVsH followed by monitoring of the morbidity rate (neuroinvasiveness test).