Table 2. The amino acid substitutions of E protein of TBEV strains (290 viruses were analyzed) that resulted in the change of net charge and/or hydrophobicity of virion surface E-gene.
| Mutation | TBEV strain | Accession No | TBEV subtype | Source of isolation | Region of isolation | Clinical manifestations (if known) |
| D67G | Est2546 | DQ393779 | FE | Field mouse | Estonia | |
| D67G | T-blood | AF091019 | FE | TBE patient | Ural | Meningoencephalitis |
| D67G | Ural-Nina | FJ214119 | FE | TBE patient | Ural | Meningoencephalitis |
| D67G | Ural-P | FJ214118 | FE | TBE patient | Ural | |
| D67G | Ural-B | FJ214117 | FE | TBE patient | Ural | |
| D67G | Ural-A | FJ214115 | FE | TBE patient | Ural | |
| D67G | Volkhov-K | FJ214114 | FE | TBE patient | Europe | Chronic encephalitis |
| D67N | RK 1424 | AF091016 | FE | I. persulcatus | Latvia | |
| E84K | 1486 | EF469755 | SIB | I. persulcatus | Siberia | |
| E84G | 4387/7 | X76608 | WE | I .ricinus | Slovakia | |
| E122G | DXAL5 | AY178833 | FE | Not specified | China | |
| E122G | KrM219 | DQ988684 | WE | Rodents | Korea | |
| E155G | 272-75 | AF231806 | SIB | Vole | Siberia | |
| E170G | Koltsovo-29 | AF540032 | FE | TBE patient | Siberia | |
| D203G | DXAL-12 | EU089977 | FE | Not specified | China | |
| D203G | DXAL-13 | EU089976 | FE | Not specified | China | |
| D203G | DXAL-21 | EU089980 | FE | Not specified | China |