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. Author manuscript; available in PMC: 2010 Aug 7.
Published in final edited form as: Cell Stem Cell. 2009 Aug 7;5(2):191–203. doi: 10.1016/j.stem.2009.05.024

Figure 5. Attenuation of Jak-STAT signaling inhibits spermatogonial dedifferentiation.

Figure 5

(A-D) Examples of GSCs (arrowheads), spermatogonia (open arrowheads) and CPC nuclei (arrow) are indicated. (A) Testes from flies misexpressing moderate levels of UAS-SOCS36E maintain GSCs, as revealed by immunostaining for GFP (green, marks nuclei of cells misexpressing SOCS36E), Armadillo and 1B1 (blue, marks the hub and fusomes, respectively), and Vasa (red, marks germ cells). (B-D) Testes immunostained for GFP (SOCS36E misexpressing cells), 1B1, marks fusomes, and Armadillo, marks the hub (green); Vasa (red), marks germ cells; and DAPI (blue). (B) Before heat-shock, testes from flies containing Hs-bam and UAS-SOCS36E have wild-type morphology; GFP is undetectable as expected. Heat-shock-induced misexpression of (C) both SOCS36E (green nuclei) and Bam or (D) Bam alone yields morphologically indistinguishable testes: GSCs are lost but CPCs (arrows) and spermatogonia (open arrowheads) remain. (E) The average number of GSCs (± standard deviation) and (F) the percentage of testes containing GSCs from testes represented in panels B-D. GSCs in testes misexpressing SOCS36E and Bam (blue) are significantly reduced (asterisks) at 5 days of recovery compared to testes misexpressing Bam alone (purple). Scale bars = 10μm.