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. Author manuscript; available in PMC: 2009 Sep 24.
Published in final edited form as: J Cell Physiol. 2009 May;219(2):503–511. doi: 10.1002/jcp.21692

Figure 7. Partial restoration of muscle differentiation by forced expression of shRNA-resistant Akt1 in C2 myoblasts lacking Akt1.

Figure 7

C2 myoblasts were infected at ~ 25% of confluent density with Ad-shAkt1 followed 24 hr later by infection either with Ad-β-gal or Ad-Akt1, and 1 day later by incubation in DM. A. Experimental scheme. B. Results showing immunocytochemistry for myogenin in nuclei (green) and for troponin-T (red) after 48 hr in DM. Magnification is 200X. B. Analysis of myogenin positive cells after 48 hr in DM (mean ± SD, n = 6; * - p < 0.001 vs. Ad-β-gal).