Inoculation scheme for generating ovalbumin-specific memory T cell responses. C57BL/6 mice (female, 6–8 weeks old) were inoculated via footpad with LV-SOCS1-siRNA-DCs, LV-GFP-siRNA-DCs, or mock transfected DCs on days 0 and 7. LPS (30 ug/mouse) was injected i.p. on days 1, 3, 8, and 10. Two to four months later, the mice were boosted with the ovalbumin peptides, OT-1 and OT-II (100 μg/mouse each), and two weeks after this boost, were analyzed for memory responses.