Table 1.
Steady-state kinetic and metal content data for FL-L1. Substrate used in the kinetic studies was nitrocefin, and kinetic studies were conducted as described in Materials and Methods
| Enzyme | kcat (s-1) | Km (μM) | Metal content (eq) |
|---|---|---|---|
| FL-L1 w/ Mna | 13 ± 1 | 5 ± 1 | 0.3Mn/0.4Fe/0.6Zn(II) |
| FL-L1 w/ Zn(II)a | 28 ± 2 | 6 ± 1 | 0.1Fe/1.9Zn(II) |
| FL-L1 w/ Fea | 3.6 ± 0.1 | 6 ± 1 | 0.9Fe/0.3Zn(II) |
| FL-L1 in LB mediumb | 26 ± 1 | 4 ± 1 | 1.9 ± 0.1 Zn(II) |
| FL-L1 in minimal mediumb | 10 ±1 | 4 ± 1 | 0.4Fe/0.3Zn(II) |
a
L1 was over-expressed in minimal medium containing 50 μM of the indicated metal ion as described in Materials and Methods.
b
L1 was over-expressed in minimal or LB medium without adding any additional metal ions.