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L1 refolded in the presence of Fe(II), Zn(II), and Mn(II). Substrate used in the kinetic studies was nitrocefin, and kinetic studies were conducted as described in Materials and Methods
| Enzyme refolded | kcat (s-1) | Km> (μM) | Metal content (eq) |
|---|---|---|---|
| w/ Zn(II) | 37 ± 1 | 3.5 ± 0.2 | 2.0 ± 0.1 Zn |
| w/ Fe(II) | < 0.1 | N/A | 2.0 ± 0.1 Fe |
| w/ Mn(II) | < 0.1 | N/A | 0.20 ± 0.05 Mn |
| w/ Zn(II) + Fe(II) |
28 ± 1 | 3.0 ± 0.2 | 1.5 ± 0.1 Zn 0.4 ± 0.1 Fe |
