Table II.
Frequency of IC50 Values Exceeding Aqueous Solubility and Assay Failure Rates for Three Fluorescence-Based CYP Inhibition Screens
| IC50/Solubility | All IC50 values | IC50 < 50 μM | IC50 > 50 μM | n a | Percentage Assay Failure Rate (n) | Failure Rate Due to Fluorescence or Fluorescence Quenching (%) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| <1 | 1–3 | 3–10 | >10 | <1 | 1–3 | 3–10 | >10 | <1 | 1–3 | 3–10 | >10 | ||||
| CYP2C9 (MFC) | 79.8 | 12.1 | 5.4 | 2.7 | 49.7 | 10.9 | 4.9 | 0.5 | 30.1 | 1.2 | 0.5 | 2.2 | 4,688 | 1.64 (5,508) | 0.80 |
| CYP2D6 (AMMC) | 71.4 | 11.5 | 10.7 | 6.4 | 49.6 | 9.8 | 9.9 | 2.3 | 21.8 | 1.7 | 0.8 | 4.1 | 4,723 | 0.94 (5,402) | 0.11 |
| CYP3A4 (DBF) | 68.6 | 12 | 9.6 | 9.8 | 25.6 | 8.6 | 7.9 | 2.6 | 43 | 3.4 | 1.7 | 7.2 | 4,747 | 0.48 (5,433) | 0.09 |
aNumber of molecules where both CYP inhibition and high throughput solubility data were available.