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. 2009 Oct;175(4):1380–1388. doi: 10.2353/ajpath.2009.090096

Figure 3.

Figure 3

EndoMT occurs in glomeruli of Tie2-Cre;LoxP-EGFP mice with 1-month DN (A–F). Confocal microscopy demonstrates DAPI (blue), EGFP (green), and α-SMA (red) in normal kidney (A) and 1-month STZ-induced DN (B) in glomeruli. In B arrow indicates an EGFP+/α-SMA+ cell in an afferent/efferent arteriole in mouse with DN. The EGFP+/α-SMA+ cell is enlarged in insets (C–E). F: Z-sections of the EGFP+/α-SMA+ cell in B. Step size = 1 μm. Confocal microscopy demonstrates α-SMA (red, G), EGFP (green, H), and DAPI (blue, merged image, I) in 6-month STZ-induced DN. Original magnification: ×1800 (A and B); ×3600 (C–F); ×600 (G–I).