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. 2009 Oct;175(4):1410–1420. doi: 10.2353/ajpath.2009.080830

Figure 3.

Figure 3

VEGF-C induces PI3K/mTOR/S6 signaling. A: Western blots to detect phospho-Akt (pAkt), phospho-mTOR (p-mTOR), phospho-S6K (pS6K), and phospho-S6 (pS6) in lysates from LDC treated with the VEGF-R3 specific agonist VEGF-C (Cys156Ser), over time in the presence or absence of wortmannin (left), rapamycin (middle), or MAZ-51 (right); total Akt, mTOR, S6K, or S6 levels served as protein loading controls. B: Immunocytochemistry of LDCs labeled for pS6 in cells expressing only GFP (top) or GFP tagged wt-TSC2 (bottom). C: Western blots to detect pAkt, p-mTOR, pS6K, and pS6 in lysates from LDC expressing GFP tagged wt-TSC2 (left four lanes) or GFP alone (right four lanes) stimulated with VEGF-R3 specific agonist VEGF-C (Cys156Ser), over time; total Akt, mTOR, S6K, or S6 levels served as protein loading controls. Bottom two panels: Western blot for TSC2 expressing GFP tagged wt-TSC2 (left four lanes) or GFP alone (right four lanes), β-actin served as protein loading control. D: Western blots to detect pAkt, p-mTOR, and pS6 in lysates from LDC expressing GFP tagged wt-TSC2 stimulated with VEGF-R3 specific agonist VEGF-C (Cys156Ser) in the absence or presence of MAZ-51 over time; total Akt, mTOR, S6K, or S6 levels served as protein loading controls.