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. 2009 Oct;175(4):1421–1430. doi: 10.2353/ajpath.2009.080845

Figure 4.

Figure 4

EMMPRIN regulates MMP-13 expression and activity in vascular endothelium. A: Immunoblot detection of ectopic FLAG-EMMPRIN, and endogenous MMP-13 in cell lysates and culture medium of FLAG-EMMPRIN expressing murine aortic endothelial cells. GAPDH was also detected for control loading purposes. B: Immunoblot detection of ectopic FLAG-EMMPRIN and endogenous MMP-13 in cell lysates and culture medium from murine aortic endothelial cells in which EMMPRIN was silenced. GAPDH was also detected for control loading purposes (n = 3 by triplicate). Right, Expression of MMP-13 in endothelial cells treated with 100 μmol/L DEA-NO in silenced-EMMPRIN cells. C: Detection of EMMPRIN in aortic rings of wild-type and MMP-13 null mice, 15 days after surgery. L: lumen. n = 3. Scale bars, 50 μm. D: 10 animals/group were either infused with elastase or saline buffer and the aneurysms (arrow) were monitored over time by magnetic resonance. Right, Graphical representation of aortic dilatation 15 days after surgery respect to sham operated mice (n = 10 mice/genotype/assay by triplicate mean ± SD; ∗P < 0.05 versus wild-type).