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. 2009 Oct;175(4):1504–1514. doi: 10.2353/ajpath.2009.081174

Figure 2.

Figure 2

Expression of CD69, CD28, and CTLA-4 on splenic CD4+ T cells after OVA immunization and in vivo OVA challenge. In this experiment, all of the animals were immunized with OVA 14 days before trauma-hemorrhage by injection of 0.1 ml of complete Freund’s adjuvant mixed with 0.1 mg of OVA. Another 0.1 mg of OVA or vehicle (PBS) was injected at the end of resuscitation in trauma-hemorrhage groups or at corresponding time points in sham groups. Twenty-four hours after the end of resuscitation, splenocytes with or without OVA challenge were isolated and stained with APC anti-CD4 Ab plus either PE Cy7-anti-CD69 Ab, PE anti-CD28 Ab, or PE anti-CTLA-4 Ab as described in Materials and Methods. Representative histograms were gated on CD4+ T cells and showed MFI of CD69 (A), CD28 (C), and CTLA-4 (E). MFI data for CD69 (B), CD28 (D), and CTLA-4 (F) are shown as mean ± SEM. *P < 0.05 versus all of the other groups. **P < 0.05 versus vehicle groups. n = 5/group. The vertical dotted line in each histogram is intended for better recognition of the shift of MFI among different treatment groups.