Figure 7.

Cytokine production capacities of splenic CD4⁺ T cells with or without OVA stimulation. Purified OVA-immunized splenic CD4⁺ T cells were cultured with mitomycin-treated macrophages with or without OVA challenge as described in Materials and Methods. After 3 days of culture, cytokine concentrations of culture supernatant were determined with cytometric bead arrays. A: Concentrations of IL-2, IFN-γ, IL-4, and IL-5. B: ratios of IL-2 or IFN-γ to IL-4 or IL-5. Data are shown as mean ± SEM. *P < 0.05 compared with other groups in A. **P < 0.05 compared with sham animal of the same group in B. n = 5/group; ND, nondetectable.