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. 2009 Oct;175(4):1638–1652. doi: 10.2353/ajpath.2009.090384

Figure 6.

Figure 6

In vitro characteristics of PC-lo/diss and PC-hi/diss cell variants. A: Adhesion of PC-lo/diss and PC-hi/diss cells to type I collagen, fibronectin, or Matrigel. Data are presented as fold differences of PC-lo/diss adhesion in comparison with adhesion levels of PC-hi/diss, determined from 3 independent experiments performed in triplicate. B: Chemotactic migration of PC-lo/diss and PC-hi/diss cells in Transwells was induced by serum-free CM from CEFs or HMVECs placed into the outer chamber. C: Haptotactic migration of PC-lo/diss and PC-hi/diss cells in Transwells was stimulated by type I collagen coated onto undersurface of the membrane. D: Matrigel invasion of PC-lo/diss and PC-hi/diss cells induced by CM from CEFs placed into the outer chamber. Data are presented as means ± SEM of cells recovered from individual outer chambers following a 48 hour migration or invasion in three independent experiments performed in duplicate. *P < 0.05, **P < 0.01, and ***P < 0.001 in two-tailed Student’s t-test. E: Western blot analysis of PC-lo/diss, PC-hi/diss and HT-1080 cell lysates (20 to 50 μg/lane) for E-cadherin, N-cadherin, and vimentin. Position of molecular weight markers is indicated in kDa on the left.