Figure 2.

Immunofluorescence of cultured monolayers immunostained for α-catenin, β-catenin, and E-cadherin. A: The DLD-1 parent and PC7 clone show extensive α-catenin and β-catenin immunostaining, localized to cell-cell junctions (arrowheads). L2a cells lack detectable α-catenin, and their β-catenin is disorganized and primarily cytosolic (arrows). Merged images show colocalization of α- and β-catenin as yellow fluorescence. A DAPI counterstain was used to identify cellular nuclei. Scale bar = 50 μm. B: The DLD-1 parent and PC7 cells express E-cadherin at cell-cell junctions (arrowheads), whereas L2a cells have dispersed E-cadherin. A DAPI counterstain was used to identify cellular nuclei. Scale bar = 50 μm.