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Effect of the calpain inhibitor ALLN on apoA-I secretion. HepG2 cells were pre-incubated with 26 μmol/L of ALLN for 30 minutes. and then with PI or DLPC (12 μmol/L) for 24 hours. ApoA-I was quantified in the media by ELISA. ApoA-I secretion is presented relative to total cell protein values and are expressed as mean ± SEM of three independent experiments; *P < 0.001 vs PI, **P < 0.001 vs DLPC.
