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. Author manuscript; available in PMC: 2010 Jan 28.
Published in final edited form as: J Am Chem Soc. 2009 Jan 28;131(3):985–992. doi: 10.1021/ja804041p

Figure 1.

Figure 1

15N-[13C CSA]-13C 3D ROCSA pulse sequence. Transverse 15N coherence is created by adiabatic CP from 1H, followed by isotropic chemical shift evolution period (t1) and selective polarization transfer to 13Cα with SPECIFIC-CP.27 ROCSA25 recoupling is applied to the 13C spins, with a coherence filter (including States-TPPI hypercomplex sampling) prior to acquisition. During 13C ROCSA, 1H was decoupled with a CW field (~125 kHz nutation frequency) and 15N with rotor-synchronized π-pulses of 10 μs with XY-16 supercycling.