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. Author manuscript; available in PMC: 2009 Sep 24.
Published in final edited form as: Nat Med. 2008 Oct 5;14(10):1088–1096. doi: 10.1038/nm.1874

Figure 5.

Figure 5

Antibodies induced by FimH immunization bind human glomerular endothelium. (a, b) Double labeling of frozen sections of normal human glomeruli with purified rabbit IgG specific for the FimH mannose binding pocket (green, a) and with an antibody to CD31 (red, b). (c) Overlay of a and b (yellow; scale bars, 25 μm). (d, e) IgG with specificity for peptide P41–49, purified from rat sera after immunization with FimH, was used for indirect immunolabeling of ultrathin frozen sections of human glomeruli. The staining pattern on endothelial cell membrane (arrows) as well as within multivesicular bodies (MVB) of endothelial and epithelial (E) is compared to that of a monoclonal antibody to hLAMP-2 (e). GBM, glomerular basement membrane. Scale bar in d, 500 nm; scale bar in e, 200 nm.